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9-cis-retinal attenuate TGF-β1-induced fibrotic injury and oxidative stress in HK-2 cells. (A–C) Western blot analysis (A) and semi-quantification (B, C) of Collagen I (Col I) and α-SMA expression in HK-2 cells treated with TGF-β1 (10 ng/ml) and 9-cis-retinal (10 μM). (D) RFU bar chart for MT-1, Ex/Em = 555 nm/600 nm, n = 5. (E) RFU bar chart for ROS, Ex/Em = 488 nm/525 nm, n = 5. Rosup and Nag serve as positive/negative controls. (F) Representative fluorescent images of ROS (scale bar = 50 μm) and MT-1 (scale bar = 200 μm) staining. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Frontiers in Nutrition

Article Title: Asiatic acid from Centella asiatica alleviates renal fibrosis: coordinated modulation of the gut–kidney axis and retinol metabolism

doi: 10.3389/fnut.2026.1802674

Figure Lengend Snippet: 9-cis-retinal attenuate TGF-β1-induced fibrotic injury and oxidative stress in HK-2 cells. (A–C) Western blot analysis (A) and semi-quantification (B, C) of Collagen I (Col I) and α-SMA expression in HK-2 cells treated with TGF-β1 (10 ng/ml) and 9-cis-retinal (10 μM). (D) RFU bar chart for MT-1, Ex/Em = 555 nm/600 nm, n = 5. (E) RFU bar chart for ROS, Ex/Em = 488 nm/525 nm, n = 5. Rosup and Nag serve as positive/negative controls. (F) Representative fluorescent images of ROS (scale bar = 50 μm) and MT-1 (scale bar = 200 μm) staining. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: 9-cis-retinal (HY-W009310, MCE, USA) at 10 μM, 4-hydroxyretinoic acid (HY-125904, MCE, USA) at 90 nM, AA at 15 μg/ml and Methoxsalen (HY-30151, MCE, USA) at 50 μM were used in HK-2 following a screening process to determine the optimal concentration.

Techniques: Western Blot, Expressing, Staining